by Steven Ertelt
November 12, 2005
Tallahassee, FL (LifeNews.com) — A new device developed by researchers at Florida State University could significantly expand the amount of cells scientists can use in adult stem cell research. The device could make the more effective form of stem cell research even better than the embryonic version.
The Florida State team reports it has designed a biomedical device that will allow stem cells derived from adult bone marrow to be grown in sufficient quantities to permit far more research. The device also allows faster growth of tissues that can be transplanted into patients.
Teng Ma, an assistant professor of chemical and biomedical engineering at the Florida A&M University-FSU College of Engineering, and colleagues have created a device called a perfusion bioreactor that is designed to mimic conditions encountered by adult stem cells within the human body. The reactor bathes stem cell samples in a protein-rich liquid while also simulating the flow of the body’s circulatory system.
“Within the human body, each cell is no more than 200 micrometers from a source of nutrients,” Ma explained. “The perfusion bioreactor allows us to deliver essential nutrients to stem cells in a manner very similar to what they are used to within the body.”
By altering that flow of nutrients to the stem cells, researchers also hope to control what type of cell they ultimately will become, Ma said.
“The perfusion bioreactor can be used to reproduce mesenchymal stem cells and to direct their differentiation into bone, cartilage, muscle, heart muscle, fat or nerve tissue,” Ma said. “The tissues grown then will be suitable for clinical transplantation.” He added that stem cells can live for up to 40 days within the bioreactor.
Ma’s research has attracted attention on several fronts.
He has received research funding totaling about $1.2 million from the Defense Advanced Research Projects Agency, the James & Esther King Biomedical Research Foundation, the American Cancer Society and the FSU Cornerstone Program. He also recently received two U.S. patents relating to the perfusion bioreactor, and indicates that they are negotiating with a technology company to manufacture the device for other stem cell researchers.
Collaborating with Ma on his perfusion bioreactor research were post-doctoral student Feng Zhao and former graduate student Warren Grayson.
While much of the controversy surrounding stem cell research has centered on the use of cells derived from fetal or embryonic tissue, Ma points out that the mesenchymal stem cells used in his research come from adult donors.
“The National Institutes of Health helped establish the Tulane Center for Gene Therapy at Tulane University as a national distributor of these cells to researchers,” he said. “The center is the source of the stem cells we use.
“All of their donors are adults between the ages of 19 and 49. Essentially, each donor undergoes a medical procedure in which a small amount of bone marrow is extracted from his or her pelvic bone.”
Within that extracted bone marrow, only about one in every 100,000 cells is a stem cell, Ma said. “Because they are so rare, the ability to reproduce stem cells in a laboratory becomes particularly significant for further research and clinical trials.”